产品货号：
GS4457
中文名称：
重组大鼠GM-CSF
英文名称：
Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
General Description
Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) is secreted by a number of different cell types(including activated T cells,B cells,macrophages,mast cells,endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation.It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages.GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate.Additionally,it can stimulate the proliferation of a number of tumor cell lines,including osteogenic sarcoma,carcinoma and adenocarcinoma cell lines.It is reported that GM-CSF has no biological effects across species.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at-20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 14.5kDa,a single non-glycosylated polypeptide chain containing 127 amino acids.
Purity
> 98% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01ng/ml,corresponding to a specific activity of>1.0×108 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS,pH7.4.
Endotoxin
Less than 1 EU/μg of rRtGM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL
TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK
相关搜索：重组大鼠GM-CSF，GM-CSF, Rat
Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) is secreted by a number of different cell types(including activated T cells,B cells,macrophages,mast cells,endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation.It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages.GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate.Additionally,it can stimulate the proliferation of a number of tumor cell lines,including osteogenic sarcoma,carcinoma and adenocarcinoma cell lines.It is reported that GM-CSF has no biological effects across species.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at-20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 14.5kDa,a single non-glycosylated polypeptide chain containing 127 amino acids.
Purity
> 98% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01ng/ml,corresponding to a specific activity of>1.0×108 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS,pH7.4.
Endotoxin
Less than 1 EU/μg of rRtGM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL
TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK
相关搜索：重组大鼠GM-CSF，GM-CSF, Rat